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Expression of Bcl-xL mRNA (A and C) and protein (B and D) in Baf-3 cells. The Northern blots were first hybridized with a mouse Bcl-x cDNA probe and then rehybridized with a human β-actin probe. (A and B) β544 cells were cultured in medium lacking either IL-3 or hGM-CSF for 8 h (lanes 2). Then cells were cultured in medium containing hGM-CSF for the indicated periods. (C and D) Baf-3 cells expressing NFIL3 were cultured in IL-3-free medium for the indicated periods.
