Figure 5.

T cell clustering following ART interruption. TCR CDR3 sequence similarities were computed using the Hamming distance to tests two pairwise CDR3s of equal length at a time creating a CDR3 similarity network. A Hamming distance of 1 reflects a single AA change between two neighboring CDR3s. (A) The distribution of pairwise Hamming distances between CDR3s in the memory CD8⁺ T cell repertoires. (B) TCR CDR3 sequence similarities were also computed using a shared triplet metric of closely related CDR3s forming network clusters in the memory CD8⁺ T cell population. (C) The number of nodes as a proportion of the number of total TCRs being clustered for each sample following PTI.