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. 2021 Aug 16;22(4):1186. doi: 10.3892/etm.2021.10620

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Copyright: © Zeng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Cx43-plasmid reverses the effects of miR-206 mimics on Cx43 expression in γ-radiation-induced HA-1800 cells. HA-1800 cells were transfected with mimics control, miR-206 mimics, control-plasmid or Cx43-plasmid for 24 h, and induced with 20 Gy γ-radiation for another 24 h. The cells were divided into six groups as follows: Control, irradiation, irradiation + mimics control, irradiation + miR-206 mimics, irradiation + miR-206 mimics + control-plasmid and irradiation + miR-206 mimics + Cx43-plasmid groups. Reverse transcription-quantitative PCR analysis of (A) miR-206 and (B) Cx43 mRNA expression levels in the aforementioned six groups. (C) Detection of Cx43 protein expression levels in the aforementioned six groups. ^**P<0.01 vs. control; ^##P<0.01 vs. irradiation + mimics control; ^&&P<0.01 vs. irradiation + miR-206 mimics + control-plasmid. miR, microRNA; Cx43, connexin 43.