Figure 1.

Establishment of transgenic mice. (A) Principle of tissue-specific inducible transgenic mice system. (B) Cre was detected in lung tissue by IHC staining (scale bar, 100 µm). (C) Cre was detected by IHC staining in the peripheral lung tissue and then SPC was detected by IHC staining after antibody stripping. The solid arrows indicate that Cre and SPC were co-localized in the same cell. (D) SPC was detected by IHC staining and then mEGFP was detected by IHC staining after antibody stripping. The solid arrows indicate that SPC and mEGFP were co-localized in the same cell, while the hollow arrows indicate that SPC and mEGFP were not co-localized in the same cell (scale bars, 50 µm). IHC, immunohistochemistry; mEGFP, cell membrane-localized enhanced green fluorescence protein; SPC, surfactant protein C; ACTB, β-actin.