Skip to main content
. 2021 Aug 12;11:699749. doi: 10.3389/fonc.2021.699749

Figure 4.

Figure 4

METTL14 regulates PTEN mRNA expression via m6A modification in STAD. (A) M6A modification level of total RNA was detected after METTL14 overexpression in HGC-27 and AGS cells. (B) Enrichment of m6A in PTEN was detected after METTL14 overexpression in HGC-27 and AGS cells (***P < 0.001). (C, D) RIP-qPCR assay were used to detect the enrichment of METTL14 binding PTEN in HGC-27 and AGS cells (*, vs IgG, P < 0.05; #, vs anti-METTL14 in NC group, P < 0.05). (E) Luciferase reporters of wild type and mutation PTEN were constructed. (F, G) Relative luciferase activity were detected after transfecting wt-PTEN 3′-UTR-Luc or mut-PTEN 3’-UTR-Luc reporter vectors in METTL14 overexpression or negative control STAD cells *P < 0.05; ns, no significant difference. (H) METTL14 overexpression mediating PTEN upregulation was antagonized by the PTEN inhibitor, VO-Ohpic, in HGC-27 and AGS cells. (I, J) The overexpression of METTL14 extended the half-life of PTEN mRNA in HGC-27 and AGS cells. *P < 0.05.