Figure 1.

Specific memory T-cell response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 102 patients who had recovered from SARS-CoV-2 infection. (A) interferon-γ (IFN-γ) (n = 102) and granzyme B (n = 68) production in response to anti-CD3 (positive control, C+) and to SARS-CoV-2 were measured by ELISpot assay. Peripheral blood mononuclear cells (PBMCs) were stimulated with anti-CD3 (1 ng/mL) or SARS-Cov-2 peptides (S1, S2, and N peptide pools) (2 µg/mL) for 18 hours at 37°C. Examples of spots generated in negative controls (C−), positive controls (C+), and in the presence of SARS-CoV-2 are shown. (B) Histograms represent the number of antigen-specific spot-forming cells producing IFN-γ and granzyme B after background subtraction of control wells with no antigen in each patient. (C) Relationship between IFN-γ and granzyme B production. Pearson correlation coefficients and probabilities are shown in the upper left-hand corner.