Fig. 2.

Avasimibe induced G1 phase cycle arrest and altered the G1 phase-related protein levels in PCa cells. a Flow cytometric analysis to detect the cell cycle for the 48 h avasimibe-treated PCa cell lines; b quantitative results of DU 145 cell fractions; c quantitative results of PC-3 cell fractions; d immunofluorescence staining of E2F-1 and Ki67 in PCa cells (DU 145 and PC-3) after avasimibe treatment; e protein levels of G1 phase-related proteins (CDK2/4/6, Cyclin D1/A1 + A2, p21 and E2F-1) in 48 h avasimibe-treated PCa cells were estimated by Western blotting. **p < 0.01, ***p < 0.001, the scale bar is 100 μm