TABLE 1.
General genomic features of the “Ca. Aenigmarchaeota” MAGs reconstructed from hot spring metagenome sequencing
| Bins | DRTY-6_1 bin 65 | DRTY-6_2 bin 201 | DRTY-6_2 bin 202 | DRTY-6_2 bin 215 | DRTY-7_1 bin 34 | GMQ_1 bin 18-1 | JZ-2_2 bin 245 | QQ_2 bin 128 |
|---|---|---|---|---|---|---|---|---|
| Genome size (Mbp) | 0.75 | 0.71 | 0.69 | 0.55 | 0.82 | 0.55 | 0.52 | 0.64 |
| GC content (%) | 31.6 | 30.9 | 28.6 | 25.7 | 29.1 | 25.5 | 47.9 | 34.2 |
| N 50 | 394,136 | 119,657 | 15,097 | 31,689 | 45,374 | 62,116 | 24,348 | 157,870 |
| No. of scaffolds | 19 | 9 | 69 | 25 | 27 | 19 | 25 | 8 |
| Completeness (%)a | 96.3 | 92.6 | 98.1 | 94.4 | 90.7 | 94.4 | 96.3 | 94.4 |
| Contaminationb | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.93 |
| Strain heterogeneityb | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| No. of RNAs | 42 | 20 | 35 | 22 | 43 | 47 | 28 | 34 |
| 5S rRNAs | 2 | 1 | 1 | 0 | 1 | 1 | 1 | 0 |
| 16S rRNAs | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 23S rRNAs | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
| tRNAs | 39 | 17 | 33 | 21 | 40 | 44 | 25 | 32 |
| No. of protein-coding genes | 865 | 729 | 840 | 662 | 923 | 662 | 637 | 757 |
| Avg length (bp) | 817 | 857 | 754 | 789 | 820 | 790 | 747 | 747 |
| Coding density (%) | 93.9 | 88.0 | 92.1 | 94.5 | 92.8 | 94.4 | 92.3 | 88.0 |
| Overlapped genes | 223 (25.8%) | 50 (6.9%) | 193 (23%) | 113 (17.1%) | 229 (24.8%) | 202 (30.5%) | 105 (16.5%) | 154 (20.3%) |
| No. of genes annotated by COGc | 392 (43.2%) | 422 (56.3%) | 382 (43.7%) | 367 (53.6%) | 451 (46.6%) | 336 (47.3%) | 350 (52.6%) | 383 (48.4%) |
| No. of genes annotated by KOc | 313 (34.5%) | 348 (46.4%) | 324 (37%) | 321 (46.9%) | 353 (36.5%) | 279 (39.3%) | 287 (43.2%) | 321 (40.5%) |
a
Genome completeness was calculated as the percentage of detected marker genes among 54 conserved single-copy genes as listed in Data Set S1 in the supplementary material.
b
Genome quality, including contamination and heterogeneity, were estimated by CheckM (16).
c
Functional annotation was conducted by uploading genomes to the IMG database.