Fig. 2.

The advanced design of CAR T cells (A) Tandem CAR: A CAR structure which design to target two tumor antigens with a distinct ectodomain (scFv) linked sequentially with a single transduction domain; (B) dual CAR: CAR structure comprising two distinct antigen-recognition domains targeting different tumor antigens linked sequentially with a single transduction domain; (C) split, universal, and programmable CAR: a specific CAR model which has designed to improve CAR programmability comprising universal receptor zipCAR that allows a vast range of tumor antigens to be targeted via different zipFv. CAR, Chimeric antigen receptor; scFv, single-chain variable fragment.