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. 2021 Aug 29;9(8):e003100. doi: 10.1136/jitc-2021-003100

Figure 1.

Figure 1

CXCR6 is highly expressed on CD8+ T cells in tumor tissues. (A) t-SNE plot showing immune cell clusters (right) and expression of CXCR6 (left) from human CRC. (B) Violin plot showing expression of CXCR6 on cell clusters from human CRC. (C) t-SNE plot showing immune cell clusters (right) and expression of CXCR6 (left) from MC38 mouse model. (D) Violin plot showing expression of CXCR6 on immune cell clusters from MC38 mouse model. (E) Violin plot showing expression of CXCR6 in PBMCs (P), tumor (T) and adjacent normal (N) tissues from patients. (F) Violin plot showing expression of CXCR6 in lymph node (LN) and tumor (T) tissues from MC38 mouse model. (G and H) Kaplan-Meier survival curves comparing the high and low expression of CXCR6 (G) and scatterplots of correlation between CXCR6 and CD8a expression (H) in TCGA database determined by gene expression profiling interactive analysis website (GEPIA, http://gepia.cancer-pku.cn/). (I) Immunofluorescence staining showing DAPI (blue)/CD8 (green)/CXCR6 (red) and their overlay in tumor tissues from human CRC samples. (J–L) Representative plots and frequencies of CXCR6+CD8+ T cells in peripheral blood (PB), lymph node (LN), spleen and tumor from normal or MC38 tumor-bearing mice (n=3–7). Error bars represent SEM. **P<0.01 and ***p<0.001. Single-cell RNA sequencing data were analyzed on website http://crcleukocytecancer-pkucn/. CXCR6, C-X-C motif chemokine receptor 6; NS, no significance.