Figure 4.

Intratumoral CXCR6⁺CD8⁺ T cells are pivotal for antitumor efficacy. (A) Set-up of bone marrow chimeric mice. Total bone marrow obtained from naïve CD45.1⁺ mice were mixed in 1:2 ratio with that from CD45.2⁺ WT or Cxcr6^−/− mice and then were injected into lethally irradiated Rag1^−/− recipient mice. Eight weeks later, MC38 cells were injected subcutaneously into chimeras. (B) Quantification of CD45.1 and CD45.2 positive cells from peripheral blood (PB) and tumor gated on CD8⁺ cells. (C) Quantification of CD45.1 and CD45.2 positive cells from tumors gated on effectors-producing CD8⁺ T cells. (D) Immunofluorescence staining of DAPI (blue)/CD8 (green)/CXCR6 (red) and their overlay in MSS and MSI tumor tissues. (E) Heatmap showing the relative expression of genes in intratumoral CXCR6⁺CD8⁺ T and CXCR6⁻CD8⁺ T cells. data are presented as the mean±SEM. *P<0.05, **p<0.01, and ***p<0.001. MSI, microsatellite instability; MSS, microsatellite stability.