FIG 6.

Lactobacillus species display antagonism with C. albicans in vitro. To observe inhibitory effects of Lactobacillus against C. albicans biofilm formation, C. albicans and a panel of Lactobacillus species were cocultured in THB/RPMI (1:1) medium in 5% CO₂ for 24 h (a), or C. albicans was grown for 4 h prior to addition of Lactobacillus species for 20 h (b). C. albicans biofilm-associated gene expression was measured in the presence of L. rhamnosus, which is associated with health, and L. iners, which is hypothesized to indicate dysbiosis. The mean log fold change relative to single species C. albicans biofilms is shown (c). Data are means and SD.