FIG. 3.

Autoradiograph of an 8% polyacrylamide gel showing protein-DNA complexes (C) between the indicated amounts of HMGI protein and the wild-type (wt) or mutated (mutI.g or mutI.cg) 5′-end-labelled double-stranded DNA sequences of the upstream HMGI binding site. The incubation reaction was carried out as described in Materials and Methods in the presence of 12.5 μg of nonradioactive sonicated salmon sperm DNA per ml as nonhomologous competitor DNA.