FIG 1.
Hydroxyurea at 25 mM causes cell elongation in B. subtilis and SOS induction after 2 h. (A) Significant SOS induction and RecA filamentation after 2 h of HU treatment. The table shows the percentage of SOS-induced (TagC-GFP) cells, mean cell lengths, and the abundance (expressed as a percentage) of RecA-GFP foci. (B) Cell length histograms for control and HU-treated cells after 1 h and 2 h. HU-treated cells are significantly longer than control-treated cells at 1 and 2 h (P, <2.2 × 10−16 by the Wilcoxon rank sum test). The dashed line marks the mean length of control-treated cells at 2 h (4.48 μm).