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Genes with decreased fitness in Tn-seq with HU treatment. (A) Experimental scheme of cell growth and treatment for Tn-seq. (B) Follow-up analysis of individual gene disruptions or gene deletions in a spot titer assay. Individual gene interruptions (gene::erm) and in-frame deletions (Δgene) were chosen for a subset of the top gene hits from growth periods 2 and 3 in Tn-seq.
