TABLE 3.

Summary of top six gene hits from Tn-seq validated using spot titer assays with resulting phenotypes on HU plates^a

Mutant	Function
ackA::erm	Acetate kinase
comEB::erm	dCMP deaminase
ΔddcA	DNA damage checkpoint agonist
ΔyaaA	Putative ribosome assembly factor
ΔybbP	Diadenylate cyclase
ΔytoI	Putative transcriptional regulator

^aGene hits were identified from Tn-seq growth periods 2 and 3. The complete gene validation list is presented in Table S5 in the supplemental material. The complete Tn-seq data sets are presented in Tables S3 and S4 (Excel files).