FIG 4.

Functional analysis of gut microbiota restructuring during FMT. (A) Volcano plots showed differences in KEGG orthology (KO) groups) content between two sets of read categories produced by the RECAST algorithm: colonizers and noncolonizers or resistant and suppressed. The x axis denotes log₂ fold change of KO RPK value produced by the HUMAnN2 pipeline. The y axis denotes adjusted P value obtained using Wilcoxon signed-rank test with FDR correction for multiple testing as a result of comparing the KO level between the same read categories. (B) The KO groups differentially distinguish read categories. The KO groups with negative log₂ fold change are associated with noncolonizers and suppressed, while colonizers and resistant KOs have a positive effect size. (C) Distribution of antibiotic and nisin resistance genes in read categories over time. The related metagenomic sample for all categories is indicated above the graphs. The antimicrobial group of genes is indicated to the left of the graphs. The read categories are indicated by different colors. The following abbreviations have been adopted for antibiotic resistance gene groups: TET, tetracyclines; MLS, macrolide, lincosamide and streptogramin; AMG, aminoglycosides.