TABLE 1.
Bacterial strains and plasmids
| Species, strain, or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5α | F− ϕ80ΔlacZM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17(rK− mK−) | Invitrogen |
| S17-1λpir | Tpr Strr recA thi pro hsdR hsdM+ RP4::2-Tc::Mu::Km Tn7 λpir | 72 |
| Yersinia pestis | ||
| YP6 | CO92, pCD1− | 11 |
| YPA18 | YP6 ΔpsaEF | 18 |
| YPA260 | YP18 with pEW104 at the native site | 18 |
| YPA425 | YP6 ΔdegP | This work |
| YPA469 | YPA18 ΔdegP | This work |
| YPA476 | YPA425 with pJQ054 at the native site | This work |
| YPA350 | YP6 Δtsp | This work |
| YPA379 | YPA18 Δtsp | This work |
| YPA438 | YPA350 with pJQ050 at the native site | This work |
| YPA370 | YP6 tsp::pJQ014 | This work |
| YPA391 | YP6 degS::pJQ032 | This work |
| YPA387 | YP6 degQ::pDF001 | This work |
| YPA395 | YP6 degP::pDF004 | This work |
| YPA382 | YP6 rseP::pJQ031 | This work |
| YPA393 | YP6 ypfJ::pDF002 | This work |
| YPA388 | YP6 ypo0398::pDF003 | This work |
| YPA396 | YP6 ftsH::pDF005 | This work |
| YPA275 | YPA18 with pEW107 at the native site | This work |
| YPA277 | YPA18 with pEW108 at the native site | This work |
| YPA276 | YPA18 with pEW109 at the native site | This work |
| YPA273 | YPA18 with pEW110 at the native site | This work |
| YPA271 | YPA18 with pEW111 at the native site | This work |
| YPA317 | YPA18 with pEW112 at the native site | This work |
| YPA274 | YPA18 with pEW113 at the native site | This work |
| YPA278 | YPA18 with pEW114 at the native site | This work |
| YPA325 | YPA18 with pEW115 at the native site | This work |
| YPA267 | YPA18 with pEW116 at the native site | This work |
| Plasmids | ||
| pSR47S | Kanr, MobRP4 oriR6K sacB suicide vector | 70 |
| pPROBE-AT | Apr, gfp reporter vector | 73 |
| pWKS30 | Apr cloning vector | 74 |
| pEW102 | psaA promoter in pPROBE-AT | 18 |
| pEW104 | psaEF and flanking sequences in pSR47S | 18 |
| pEW105 | psaEF promoter and psaE in pSR47S | 18 |
| pEW106 | psaEF promoter and psaF in pSR47S | 18 |
| pPsaF | psaEF promoter and psaF coding sequence in pWKS30 | 18 |
| pJQ014 | pSR47S with an internal fragment from YP6_1705 (tsp) | This work |
| pJQ032 | pSR47S with an internal fragment from YP6_ 3568 (degS) | This work |
| pDF001 | pSR47S with an internal fragment from YP6_ 3566 (degQ) | This work |
| pDF004 | pSR47S with an internal fragment from YP6_ 3382 (degP) | This work |
| pJQ031 | pSR47S with an internal fragment from YP6_ 1051 (rseP) | This work |
| pDF002 | pSR47S with an internal fragment from YP6_ 3058 (ypfJ) | This work |
| pDF003 | pSR47S with an internal fragment from YP6_ 0398 (peptidase family M48) | This work |
| pDF005 | pSR47S with an internal fragment from YP6_ 3502 (ftsH) | This work |
| pJQ018 | tsp flanking sequences in pSR47S | This work |
| pJQ047 | degP flanking sequences in pSR47S | This work |
| pJQ054 | degP coding and flanking sequences in pSR47S | This work |
| pJQ050 | tsp coding and flanking sequences in pSR47S | This work |
| pEW107 | psaEF and flanking sequences with psaEC206S mutant allele in pSR47S | This work |
| pEW108 | psaEF and flanking sequences with psaEC211S mutant allele in pSR47S | This work |
| pEW109 | psaEF and flanking sequences with psaEC206S/C211S mutant allele in pSR47S | This work |
| pEW110 | psaEF and flanking sequences with psaFH38A/H116A/H159A mutant allele in pSR47S | This work |
| pEW111 | psaEF and flanking sequences with psaFH40A mutant allele in pSR47S | This work |
| pEW112 | psaEF and flanking sequences with psaFH54A mutant allele in pSR47S | This work |
| pEW113 | psaEF and flanking sequences with psaFH87A mutant allele in pSR47S | This work |
| pEW114 | psaEF and flanking sequences with psaFH141A mutant allele in pSR47S | This work |
| pEW115 | psaEF and flanking sequences with psaFH153A mutant allele in pSR47S | This work |
| pEW116 | psaEF and flanking sequences with psaFH155A mutant allele in pSR47S | This work |
a
Str, streptomycin; Kan, kanamycin; Ap, ampicillin; r, resistance; Tp, trimethoprim.