TABLE 2.

Primers used in this study

Primer	Sequencea (5′ to 3′)	Descriptionb
psaEFcompF	ATCGATCCTCTAGAGTCGACATTAACGGGGGCGCTGTCTATGG	F pEW104 5′
psaEFcompR	GCTCTAGAACTAGTGGATCCATAACTCAGTCGCAGACCTATAG	R pEW104 3′
psaF_3HA_R	CATTCTTAAATCAAGATGCTCAGCGATATCGCCATATTTCACTTC	R pEW110 internal 3′
psaF_3HA_F	GAAGTGAAATATGGCGATATCGCTGAGCATCTTGATTTAAGAATG	F pEW110 internal 5′
psaF_H40A_R	CTTAAATCAAGAGCCTCATGGATATCGCCATATTTC	R pEW111 3′
psaF_H40A_F	GGCGATATCCATGAGGCTCTTGATTTAAGAATGCAAGG	F pEW111 5′
psaF_H54A_R	CAATAATATAAGCTGAGAGGCTAAATCTTATCCC	R pEW112 3′
psaF_H54A_F	GATTTAGCCTCTCAGCTTATATTATTGATGATAAGTCT	F pEW112 5′
psaF_H87A_R	GACTGATATGAAGCAAGAGGGAATAGGTAATACTTCC	R pEW113 3′
psaF_H87A_F	CCTATTCCCTCTTGCTTCATATCAGTCATCCCCTGAT	F pEW113 5′
psaF_H141A_R	GAGAAGATAAGGCGATCGTTTCTCCATTGACATC	R pEW114 3′
psaF_H141A_F	GGAGAAACGATCGCCTTATCTTCTCTGTTTCTCGGG	F pEW114 5′
psaF_H153A_R	CGTATGGATAGCTTCTCCTTGTACCCCGAGAAAC	R pEW115 3′
psaF_H153A_F	GGTACAAGGAGAAGCTATCCATACGTCCTATCATG	F pEW115 5′
psaF_H155A_R	CATGATAGGACGTAGCGATATGTTCTCCTTGTACCCC	R pEW116 3′
psaF_H155A_F	GGAGAACATATCGCTACGTCCTATCATGACGTTAG	F pEW116 5′
JQ076	AAAAAGGATCGATCCTCTAGAGCGCGGATAAAAAGTATTCGCTGG	F pJQ014 5′
JQ077	GCCGCTCTAGAACTAGTGGATCCCGCTATCGTTTTACTCTTCGC	R pJQ014 3′
JQ107	AAAGGATCGATCCTCTAGAGGCCAGCAGCTCCCTAGCCTGG	F pDF004 5′
JQ108	GCTCCACCGCGGTGGCGGCCGCGCCGCCGGAATTACCACGGT	R pDF004 3′
JQ110	AAAAAGGATCGATCCTCTAGAGCCCAGCGAATCGAATGGCCG	F pDF005 5′
JQ111	GCTCCACCGCGGTGGCGGCCGGACACGGGATGCACCGACAC	R pDF005 3′
VM1	GCGTCGACGATGAGCGTGTAGAGGCTGTTGCGCC	F pJQ031 5′
VM2	ATAAGAATGCGGCCGCCCCAACATGCTTACCGTCAACCGC	R pJQ031 3′
VM3	GCGTCGACCAGTACGTCGTGCCGCACCGGCGG	F pJQ032 5′
VM4	ATAAGAATGCGGCCGCCCGATGCCTTCCGGTGTTTCGCC	R pJQ032 3′
VM5	GCGTCGACGAGAGCAGCCGGCCATTCGAAGGC	F pDF001 5′
VM6	ATAAGAATGCGGCCGCGGTTTGATCCCGGCTTTCGCTGCCG	R pDF001 3′
VM7	GCGTCGACGGTCGCTATCCTGATTATTGTGTTGG	F pDF002 5′
VM8	ATAAGAATGCGGCCGCCCGATAAGCGATTACCTTCCGCCTGAG	R pDF002 3′
VM9	GCGTCGACCCACTGAACCTGACATTTCCCGATGG	F pDF003 5′
VM10	ATAAGAATGCGGCCGCGTCATCCACATAAAGGTCAAGGCGAC	R pDF003 3′
JQ080	AAAAAGGATCGATCCTCTAGAGGGTCAAAGTCGGTGCTGAGC	F pJQ050 5′
JQ083	GCCGCTCTAGAACTAGTGGATCCCGCCAGGTTGGTGAGAAGG	R pJQ050 3′
JQ081	GCGACAGGGCGAGATGCAAGCCTAGGTTGGCCTCCGTATC	R pJQ018 up 3′
JQ082	TTGCATCTCGCCCTGTCGCTGCTGCTACCGCTGGGGCAACGG	F pJQ018 down 5′
JQ134	AAAAAGGATCGATCCTCTAGAGGGGCTGCTTGATATTTATAGC	F pJQ054 5′
JQ137	CCGCTCTAGAACTAGTGGATCGGGCACAGGCTGTCAGGCAAGC	R pJQ054 3′
JQ135	ACTGTATCAATACCTTACTGCATATTCGTGTTCTCACTATAC	R pJQ047 up 3′
JQ136	GCAGTAAGGTATTGATACAGTAAGGTATTGATACAGTAAGG	F pJQ047 down 5′
psaEC206S_F	AAACAGCAATGAAAGCAAAATCACTTACTCTCATA	F pEW107 internal 5′
psaEC206S_R	CTTTCATTGCTGTTTGCATTCCGATTGATCAGAGATAATGACTAAACCAAAATC	R pEW107 internal 3′
psaEC211S_F	AAACAGCAATGAAAGCAAAATCACTTACTCTCATA	F pEW108 internal 5′
psaEC211S_R	CTTTCATTGCTGTTTGGATTCCGATTGATCACAGATAATGACTAAACCAAAATC	R pEW108 internal 3′
psaEC206-211S_F	AAACAGCAATGAAAGCAAAATCACTTACTCTCATA	F pEW109 internal 5′
psaEC206-211S_R	CTTTCATTGCTGTTTGGATTCCGATTGATCAGAGATAATGACTAAACCAAAATC	R pEW109 internal 3′

^aRestriction sites are shown in bold. Sequence overlap for Gibson assembly is underlined.

^bF, forward primer; R, reverse primer.