Analysis of biosynthesis of fatty acids. (A) Analysis of pathways from proteome and gene expression data. (B) qRT-PCR analysis of genes for biosynthesis of fatty acids. (C) Activity of ACC in the biosynthesis of fatty acids. The upregulated proteins or genes (in red) and downregulated proteins or genes (in green) are all shown in the flowchart. Results in panels B and C are displayed as mean ± SEM, and P values are identified: *, P < 0.05, and **, P < 0.01, by two-tailed Student's t test. ACC, acetyl-CoA carboxylase; FabA, 3-hydroxyacyl-[acyl-carrier protein] dehydratase; FabB, 3-oxoacyl-[acyl-carrier protein] synthase I; FabD, [acyl-carrier protein] S-malonyltransferase; FabF, 3-oxoacyl-[acyl-carrier protein] synthase II; FabG, 3-oxoacyl-[acyl-carrier protein] reductase; FabH, 3-oxoacyl-[acyl-carrier protein] synthase III; FabV, enoyl-[acyl-carrier protein] reductase; FabZ, 3-hydroxyacyl-[acyl-carrier protein] dehydratase.