The tree was constructed using 21 genomes from this study (AKU) (S2 Table) along with 58 other Pakistani and 449 full-length SARS-CoV-2 reference sequences (S1 File). AKU study sequences are indicated in blue, while other Pakistan sequences are shown in green. AKU sequences clustered with those from India (purple), Saudi Arabia (orange), United States (pink), China (light blue), Australia (turquoise), Bangladesh (yellow), France (light green) and also with other sequences from Pakistan (green). The root of the tree was determined using TreeRate tool by applying generalized midpoint rooting strategy. Nodes with significant (>0.90) aLRT-SH like support values are colored maroon. The tree was visualized and edited in Figtree software.