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. 2021 Aug 19;19(8):e3001387. doi: 10.1371/journal.pbio.3001387

Fig 8. Neutrophils from HIV-infected individuals with high CD4 T-cell count have increased IL-10 activity and decreased glycolysis.

Fig 8

(A) Representative plots and (B) cumulative data of GLUT1 and Gal-9 expression in neutrophils of HCs versus HIV patients with low or high CD4 T-cell count. (C) Cumulative data showing the MFI of GLUT1 in neutrophils from HCs versus HIV patients with low or high CD4 T-cell count. (D) Correlation of GLUT1 expression and CD4 T-cell count on neutrophils of HIV-infected patients. (E) Representative plots and (F) cumulative data of GLUT1 expression in neutrophils treated or untreated with plasma from HCs, HIV patients with low and high CD4 T-cell count. (G) ELISA results showing IL-10 concentrations in the plasma of HIV-infected individuals with low and high CD4 T-cell count and HCs. (H) GLUT1 expression in the presence or absence of IL-10 treatment and/or LPS. (I) Representative images of GLUT1 and CD11b. (J) Cumulative data showing colocalization of CD11b and GLUT1 in the presence or absence of IL-10 and/or LPS. (K) Representative plots and (L) cumulative data of ROS expression on neutrophils in the presence of IL-10 and LPS. (M) Cumulative data of Gal-9 expression on neutrophils in the presence or absence of IL-10 and/or LPS. (N) Cumulative data of Gal-9 mRNA expression and (O) shed Gal-9 in culture supernatants of LPS-activated neutrophils in neutrophils from HCs once stimulated with LPS for 3 hours in the presence or absence of IL-10 (400 pg/ml). The underlying data can be found in S1 Data. Gal-9, Galectin-9; HC, healthy control; IL, interleukin; LPS, lipopolysaccharide; MFI, median fluorescence intensity; ROS, reactive oxygen species.