Fig 3. EGFRAP downregulation increases apoptosis and JNK activity in wild-type cells adjacent to Ras^V12 expressing cells.

(A-G) Maximal projection of confocal views of wing imaginal discs from third-instar larvae expressing GFP (green) and the indicated UAS transgenes under the control of ap-Gal4, stained with anti-GFP (green), anti-Dcp-1 (red in A-C, white in A’-C’), anti-pJNK (red in E-G) and Hoechst (DNA, blue). (D, H) Box plots of mean fluorescent Dcp-1 (D) and pJNK (H) intensities of wing discs of the designated genotypes. (I-K) Confocal yz sections parallel to the A/P axis of wing imaginal discs from third-instar larvae expressing GFP (green) and the indicated UAS transgenes under the control of ap-Gal4, stained with anti-GFP (green), anti-Dlg (red) and Hoechst (DNA, blue). Apical side of wing discs is to the top. Arrows in I-K point to the border between dorsal experimental and ventral control cells. The statistical significance of differences was assessed with a t-test, **** and *** P values <0.0001 and <0.001, respectively. Scale bars, 50 μm (A-G) and 30 μm (I-K).