Skip to main content
. 2021 Aug 19;17(8):e1009738. doi: 10.1371/journal.pgen.1009738

Fig 7. EGFRAP regulates EGFR activity in Drosophila wing imaginal discs.

Fig 7

(A-C’) Maximal projection of confocal images of third instar wing imaginal discs expressing the indicated UAS transgenes under the control of en-Gal4, stained with anti-RFP (red) and anti-pERK (green). (D) Box plot of the posterior/anterior pERK intensity ratio of wing discs of the designated genotypes. (E, F) Adult flies expressing the indicated transgenes under the control of ap-Gal4. Insets in E and F show confocal images of third instar wing imaginal discs expressing GFP and EGFRAP-GFP, respectively, under the control of ap-Gal4, stained with anti-GFP (green) and anti-EGFRAP (red). (G) Maximal projection of confocal images of an EGFR-sfGFP third instar wing imaginal disc expressing EGFRAP under the control of Hh-Gal4 stained with anti-GFP (green), anti-EGFRAP (red) and Hoechst (DNA, blue). (G’-G”) Confocal yz sections, along the white dotted lines on the anterior (A) and posterior regions (P) of the wing disc shown in G. Apical side of the wing disc is to the left. The statistical significance of differences was assessed with a t-test, **** and ** P values <0.0001 and <0.01, respectively. Scale bars, 50 μm (A-C’, G).