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Recombinant MOT1 can activate or repress basal transcription driven by either the CYC1 or ACT1 promoters. (A) Basal transcription reactions were performed as described in the legends to Fig. 2 and 3, with 10 ng (lanes 2 and 9), 50 ng (lanes 3 and 10), 100 ng (lanes 4 and 11), 300 ng (lanes 5 and 12), 600 ng (lanes 6 and 13), or 1,000 ng (lanes 7 and 14) of MOT1. (B) Quantitation by phosphoimager analysis of the data shown in panel A.
