FIG. 7.

Comparison of the effects of MOT1 on HIS4 basal transcription in the presence and absence of nonpromoter DNA. Transcription reaction mixtures contained 3.4 μg of pKS II+ nonpromoter DNA as indicated and/or MOT1. (A) Transcription reactions were performed with the HIS4 core promoter with (+) or without (−) MOT1 purified from yeast. The reaction mixtures in lanes 2 and 3 contained 3 U of MOT1, those in lanes 6 and 9 contained 2 U of MOT1, and those in lanes 7 and 10 contained 8 U of MOT1. (B) Reactions were performed as described for panel A but with 250 ng (lanes 2 and 5) or 800 ng (lanes 3 and 6) of recombinant MOT1 from baculovirus.