FIG. 8.

Transcription driven by a larger (542-bp) fragment of the HIS4 promoter is repressed but not activated by MOT1. (A) Transcription reactions were performed with the plasmid containing the larger 542-bp fragment of the HIS4 promoter (lanes 1 to 9) or the HIS4 core promoter (lanes 10 to 12). The reaction mixtures in lanes 1 and 7 to 12 contained no added MOT1, whereas rMOT1 was added to the reaction mixtures in lane 2 (10 ng), lane 3 (30 ng), lane 4 (100 ng), lane 5 (300 ng), and lane 6 (1,000 ng). The reaction mixtures in lanes 8 and 11 contained 1.2 μg of pKS II+ nonpromoter DNA, and the reaction mixtures in lanes 9 and 12 contained 2.6 μg pKS II+. The bands that were quantitated are indicated by the arrow. (B) Quantitation of the data shown in panel A, lanes 1 to 6.