Figure 9.

Role of HIF-1α in morphine mediated EV release and cargoes. (A) Quantitative analysis of EV numbers isolated from condition media of HPAs transfected with either scrambled siRNA or HIF-1α siRNA followed by exposure to morphine. (B) Representative western blot images showing protein levels of Aβ mOC64 and APP in HPAs transfected with either scrambled or HIF-1α siRNA followed by exposure to morphine. Data are presented as mean±SEM. One-way ANOVA followed by Bonferroni post hoc test was performed, $P< 0.001 versus control, #P< 0.001 versus scrambled siRNA+Morphine. *P < 0.05 versus control. Abbreviations: APP- amyloid precursor protein, Aβ- amyloid beta, HIF-1α- hypoxia-inducible factor, siRNA- small interfering RNA.