Figure 2.

Comparison of EVs derived from early- versus late-passage iMSCs in a mouse model of pSS. EVs (1.5 × 10¹⁰ EVs/per mouse) derived from early- (P5) or late-passage (P15) iMSCs or vehicle control (PBS) were injected into the tail veins of NOD.B10-H2b mice twice a week for two weeks (n= 5). Two weeks after the last injection, SMG tissue and serum samples were collected. (A) Representative H&E staining of SMG tissue from NOD.B10-H2b females. (B) Quantification of the lymphocytic infiltration. The areas of lymphocyte infiltrate were quantified from three H&E stained sections from each of 5 SMGs. (C) Serum levels of anti-La and anti-Ro52 auto-antibodies were determined with ELISA. (D) Relative levels of mRNAs related to immune responses in submandibular glands were determined with qRT-PCR. All data are presented as means ± SD. ns: not significant, *: p < 0.05 by one-way ANOVA followed by Dunnett's or Tukey's test.