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. 2021 Sep 1;12(6):1409–1422. doi: 10.14336/AD.2021.0621

Figure 7.

Figure 7.

Manipulation of miR-21 or miR-125b in late-passage iMSCs improves the immune regulatory function of their EVs. (A) After transient transfection of miR-21 mimic or miR-125b inhibitor into P14 iMSCs, iEVs were isolated and levels of miR-21b and miR-125b in iEVs were determined with qRT-PCR. (B-C) The relative cell numbers and EV yields of iMSCs at 2 days after transfection with miR-21 mimic (OE) or miR-125b inhibitor (KD). (D) RT-PCR assays for TNFa and IL-12a in splenocytes stimulated with LPS for 4 hrs with or without EVs (0.75 to 3 X109 particles/ml) from late-passage (P14) iMSCs transfected with target miRNA inhibitor or mimic. (E) IFN-γ, IL-6, and IL-17 ELISAs with conditioned medium of splenocytes stimulated with LPS for 18 hrs with EVs (0.75 to 3 X109 particles/ml) derived from late-passage (P14) iMSCs transfected with target miRNA inhibitor or mimic. All data are presented as means ± SD (n=4). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons test.