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. 2021 Aug 8;12(19):5903–5913. doi: 10.7150/jca.60969

Figure 1.

Figure 1

miR-193a-5p is upregulated in osteosarcoma tissues and promotes colony formation, migration, invasion and EMT. A-C. RT-qPCR was used to detect relative level (RL) of miR-193a-5p in osteosarcoma tissues (A), cell lines (B) and the cells (C) stably expressing pre-miR-193a (193UP), anti-miR-193a-5p (193DN) and non-target sequence (Ctrl). D. Representative images of colony (left panel) and the colony numbers (right panel). Bar length: 1 cm. E. Representative images (left panel) of wound area at the indicated time and percentage of wound closure (right panel). Bar length: 20 µm. F. Representative images (left panel) and numbers (right panel) of invaded cells in the Transwell invasion assay. Bar length: 20 µm. G. The influence of miR-193a-5p on EMT-related marker was determined by Western blotting. All the values were presented as means + SD for three independent experiments. Differences between two groups were analyzed by student t-test; * p ≤ 0.05, ** p ≤ 0.01.