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. 2021 Aug 8;12(19):5903–5913. doi: 10.7150/jca.60969

Figure 3.

Figure 3

miR-193a-5p negatively regulates the expression of NCX2 gene. A. Western blotting was performed to detect the influence of miR-193a-5p on the expression of HLA-B and NCX2. B. Diagram of the binding between miR-193a-5p seed sequence and the 3′-UTR of NCX2. The mutated nucleotides in the pmirGLO-mtMRE construct were underlined. C. Each luciferase construct (pmirGLO-MRE, pmirGLO-mtMRE) was co-transfected with miR-193a-5p mimics or negative control (miR-NC) into HEK293 cells. At 24 h post-transfection, the luciferase activity was examined and normalized to Renilla luciferase activity. D. Western blotting was used to detect the expression of NCX2 in 6 pairs of osteosarcoma (T) and the matched adjacent non-cancerous (N) tissues. E. RT-qPCR was performed to detect the mRNA level of NCX2 in 25 pairs of osteosarcoma and the matched adjacent non-cancerous tissues. F-G. The correlation between NCX2 level and miR-193a-5p (or miR-193a-3p) in osteosarcoma tissues.