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. 2021 Jul 25;12(19):5712–5722. doi: 10.7150/jca.58979

Figure 4.

Figure 4

lncRNA-H19 sponged miR‑29b‑3p in EOC cells. A) lncRNA-H19 is targeted by miR‑29b‑3p; B-C) Luciferase activities in the SKOV3 and SKOV3-CB cell lines were compared between different transfection groups. * P<0.05 vs. the miR-29b-3p mimic+H19-Mut group; D) Influence of miR‑29b‑3p on lncRNA-H19 expression was determined in the SKOV3 and SKOV3-CB cell lines; E) Influence of lncRNA-H19 on miR‑29b‑3p expression was determined in the SKOV3 and SKOV3-CB cell lines; F) Ago2-RIP followed by miRNA RT-qPCR to detect the endogenous miR-29b-3p association with the Ago2-tagged lncRNA-H19 transcript; G) RNA pull-down assays were performed in EOC cells. lncRNA-H19 levels were measured by RT-qPCR. * P<0.05 vs. the negative control. miR/miRNA, microRNA; si, short interfering RNA; NC, negative control; ncRNA, noncoding RNA; lncRNA, long noncoding RNA; RIP, RNA binding protein immunoprecipitation.