Figure 6.
Knockdown of TMEFF1 inhibits the migration and invasion of Ishikawa and HEC-1B cells. (A) Expression of TMEFF1 in endometrial carcinoma cell lines. TMEFF1 (Molecular Weight): 41kDa; GAPDH (Molecular Weight): 36kDa. (B and C) After TMEFF1 knockdown in the intimal cancer cell lines Ishikawa and HEC-1B, the expression of TMEFF1 mRNA and protein was determined. (D and E) Scratch test to determine the effect of TMEFF1 expression on the migration of Ishikawa and HEC-1B cells. (F and G) Transwell assay assessing the effect of TMEFF1 expression on the invasion of Ishikawa and HEC-1B cells. Data are presented as the mean ± SEM (n = 3 per group).**P < 0.01 and ***P < 0.001.