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. 2021 Aug 17;12(19):5977–5986. doi: 10.7150/jca.54528

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CAP selectively triggers cell apoptosis in prostate cancer cells. (A) Flow cytometry images showing cell apoptosis rate in response to CAP treatment. Treat the medium for 5 minutes, and then measure the apoptotic rate after 8 hours of incubation. (B) Expression of apoptosis related proteins caspase 7 and Bcl-2 after PAM treatment. (C) Standard quantification of the expression of apoptosis related proteins caspase 7 and Bcl-2. PNTA1 and DU145 are normal prostate cells and prostate cancer cells respectively. CAP represents cold atmospheric plasma. The medium was under CAP exposure for 5 minutes, and the protein was extracted after 8 hours of incubation. Three independent replicates were performed in western blot.