Figure 6.

Alterations of intracellular ROS levels in PNT1A cells and DU145 cells receiving different CAP doses. (A) Intracellular ROS levels and (B) quantification in PNT1A and DU145 cells under different CAP doses (high dose is 1.2 kV and 5 minutes of CAP treatment; low dose is 1.0kV and 3minutes of CAP treatment). (C) Representative scheme showing ROS-associated CAP selectivity on prostate cancer cells. PNTA1 and DU145 are normal prostate cells and prostate cancer cells respectively. Percentage of G₀/G₁ stage after removing each RONS component in (D) prostate cancer cells DU145 and (E) normal cells PNT1A. Hydroxyl radical (OH·), hydrogen peroxide (H₂O₂), ozone (O₃), superoxide anion (O^2·-), nitric oxide (NO·), and electron (e^-) are quenched by mannitrol, uric acid, tiron, hemoglobin and monopotassium phosphate, respectively. CAP represents cold atmospheric plasma. Three independent replicates were performed.