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Endogenous and plasmid-encoded telomerase RNA genes in transformants. Southern blots of total DNA (1 μg), isolated from transformants and digested with SacI plus XbaI, were probed with the radiolabeled P. tetraurelia TER gene. The endogenous and plasmid-encoded gene fragments migrate to 4.4 and 0.7 kb, respectively. All transformants analyzed included the TER gene as indicated. Analyses of C52A and C52U transformants are not shown. w.t., wild type.
