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Southern blots of telomeres from transformants. Total DNA (8 μg) was digested with DraI and separated on 0.8% agarose gels (in quadruplicate) prior to capillary transfer of the DNA to Nytran filters. The radiolabeled oligonucleotides used as probes are as indicated. Telomerase RNAs expressed in transformants are as follows: lane 1, wild type; lane 2, A51G; lane 3, A50G; lane 4, A50G A56G; lane 5, C52G.
