Fig. 4.

Overexpression of ACAA1 p.N299S via adeno-associated virus delivery exacerbated amyloid-β (Aβ) pathology. APP/PS1∆E9 mice and WT littermates were injected with AAV-Vector, AAV-ACAA1 WT, or AAV-ACAA1 N299S at 3 months of age and sacrificed at 9 months of age. a Fluorescent signals in the brain section of a 9-month-old mouse receiving AAV-Vector, AAV-ACAA1 WT, or AAV-ACAA1 N299S. b Quantification of soluble and insoluble Aβ40 and Aβ42 in the hippocampus and cortex tissues of mice in a by ELISA. c, d Representative microphotographs of hippocampal sections stained with an 4G8-specific Aβ antibody (c), and quantitative analysis of the number of Aβ plaques shown by 4G8 immunoreactivity in hippocampal and cortex tissues in APP/PS1ΔE9 mice (d). Bars represent mean ± SD. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; Student’s t test