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. 2021 Aug 31;11:17466. doi: 10.1038/s41598-021-96970-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of ANP treatment on HFD-induced enlargement of lipid droplets and inflammation in adipose tissues and hepatic steatosis. (a) Representative histological images (hematoxylin–eosin staining) of each tissue harvested from the indicated mice at three weeks after treatment with or without ANP (n = 3 each). The images were captured at a field lens magnification of × 4 (left, bars = 500 μm) and × 20 (right, bars = 100 μm). The arrow heads indicate crown-like structures. (b) The number of large lipid droplets (> 100µm²)/field in BAT (field lens × 20, n = 3 each). (c) The cell area/field in iWAT (field lens × 20, n = 3 each). (d) The number of crown like structures/field in eWAT (field lens × 4, n = 3 each). (e) The number of large lipid droplets (> 100µm²)/field in liver (field lens × 20, n = 3 each). NAFLD score and steatosis score calculated in each group (n = 3 each). Triglyceride content in the hepatic tissues (n = 5 each). Data are mean ± SEM. *P < 0.05 and **P < 0.01 versus NFD; ^†P < 0.05 and ^††P < 0.01 versus HFD; ^ǂP < 0.05 and ^ǂǂP < 0.01 versus NFD + ANP.