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. 2021 Aug 31;11:17466. doi: 10.1038/s41598-021-96970-9

Figure 4.

Figure 4

Effects of ANP treatment on UCP1 expression in adipose tissues. (a) Representative images of UCP1 immunostaining in each adipose tissue harvested from the indicated mice at three weeks after treatment with or without ANP (n = 3 each). The images were captured at a field lens magnification of × 4 (left, bars = 500 μm) and × 20 (right, bars = 100 μm). (b) The relative mRNA expression of UCP1 in each adipose tissue harvested from the indicated mice at three weeks after treatment with or without ANP (n = 5 each). The qPCR data were normalized to GAPDH. The data are shown as the fold change normalized to the levels found in NFD group. Data are mean ± SEM. *P < 0.05 and **P < 0.01 versus NFD; †P < 0.05 versus HFD; ǂP < 0.05 and ǂǂP < 0.01 versus NFD + ANP. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; UCP1, uncoupling protein 1.