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. 2021 May 7;28(9):2778–2796. doi: 10.1038/s41418-021-00784-1

Fig. 6. CTX-mediated tumoricidal activity is affected by the gut microbiota composition in avatar AT3 bearers.

Fig. 6

A. Experimental Setting. As in Fig. 5, FMT using stools from HV or early BC was performed following 3 days of ATB in specific pathogen-free (SPF) C57BL/6 mice. Two weeks later, AT3 breast tumors were subcutaneously inoculated. 9 days after, mice were randomly assigned to cohousing or FMT with different donors (HV or BC) and concomitantly treated with 4 intra peritoneal (i.p.) injections of cyclophosphamide (CTX). Tumor size was recorded for each mouse on days D7, D14, D21 after CTX starting. 5–6 mice/groups for each FMT and each donor. B–D. Means AT3 tumor sizes ± SEM at different timepoits (D7, D14, and D21 after CTX starting) showing the BC induced dysbiosis and compensation with FMT or cohousing with different HV (described in Fig. 5) resulting in rescued response to CTX (each color representing one donor, details on donors charactheristics are showed on Fig. 5B). Each experiment included 5–6 mice per group. ANOVA statistical analyses of means and SEM: *p < 0.05, **p < 0.01, ***p < 0.001, ns: non-significant.