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. 2021 Sep 1;8:195. doi: 10.1038/s41438-021-00630-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Phylogenetic analysis of TabHLH1 and related bHLH TFs. B Sequence alignment of TabHLH1 sequences in T. antungense. TabHLH1 (in this article, QBQ52949.1; Taraxacum antungense bHLH1), GhbHLH1 (CAA07615.1; Gerbera hybrid cultivar bHLH1), GbbHLH1 (BAJ17663.1; Gynura bicolor bHLH1), AabHLH1 (PWA53551.1; Artemisia annua bHLH1), DpbHLH (BAJ33516.1; Dahlia pinnata bHLH), CcbHLH1 (KVH95430.1; Cynara cardunculus var. scolymus bHLH1), CsbHLH1 (AOY10782.1; Camellia sinensis bHLH1), RgbHLH1 (ATZ76571.1; Rubus genevieri bHLH1) and AtbHLH1 (AAB72192.1; Arabidopsis thaliana bHLH1). The conserved domains pfam14215 (bHLH-MYC-N) and pfam00010 (HLH) are indicated by straight lines. The neighbor-joining method in MEGA software (version 8.0) was used to construct the phylogenetic tree. Bootstrap percentage (1000 replicates) values are listed above the branches. The TabHLH1 protein is labeled with a red circle. The black line indicates the bHLH-MYC-N motif; pfam14215. The red line indicates the HLH motif; pfam 00010. C Subcellular localization of the TabHLH1 protein in N. benthamiana leaves. Tobacco leaf cells were transiently transformed with recombinant plasmids containing TabHLH1-YFP or YFP (control group). DAPI staining indicates localization in the nucleus