Figure 4.
KDM5B and RBBP5 are potential downstream targets of ALKBH5 that regulate the cardiac differentiation of MESs
(A) GO analysis based on m6A transcriptomic array. (B) qRT-PCR analysis of CTBP2, DMAP1, SFI1, KDM5B, and TGIF1 in control and ALKBH5 OE cells at day 3 of CM differentiation. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.005 (mean ± SEM; n = 4). (C) qRT-PCR analysis of KDM5B, MLL1, ASH2L, WDR5, and RBBP5 in control and ALKBH5 OE cells at day 3 of CM differentiation. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.005 (mean ± SEM; n = 4). (D) Western blot assay of RBBP5 and KDM5B on day 3 in control and ALKBH5 OE cells. β-actin was used as a loading control. (E) Western blot assay of RBBP5 and KDM5B on day 3 in control and ALKBH5 KD cells. β-actin was used as a loading control.