Figure 5.

PRRs are genetically required for the regulation of BBX19 on circadian period. A, B, Circadian rhythm of CCA1:LUC was measured in the bbx19-3 prr5-1, bbx19-3 prr7-3, bbx19-3 prr9-1, and bbx19-3 prr5-1 prr7-3 knockout mutants. Arabidopsis seedlings were grown under 12:12 LD cycles, 22°C, for 7 days before transferred to LL for luminescence measurement. The circadian parameters analysis was performed using the fast Fourier transform-nonlinear least squares based on LL24-120 rhythmic traces (A). Period estimation for individual seedlings is plotted against their relative amplitude errors (RAEs value the robustness of rhythmicity) (B). C, Period length estimation of CCA1:LUC circadian rhythm (B). Multiple groups were analyzed with one-way ANOVA followed by Tukey’s multiple comparison test, P < 0.05. D–F, Circadian rhythm of CCA1:LUC was measured in the bbx19-3 cca1-1, bbx19-3 lhy-20, bbx19-3 cca1-1 lhy-20, and bbx19-3 toc1-101 mutants