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. 2021 Jun 4;33(8):2662–2684. doi: 10.1093/plcell/koab158

Figure 4.

Figure 4

Atatr and Atatm mutants display similar sensitivity to zeocin and bleomycin, both DSB inducers. A, Representative seedlings after growth for 12 days under control conditions (untreated) or in the presence of 5.0-µM zeocin or 0.2 µg/mL bleomycin (BLM). Scale bar = 3.0 cm. B, Root length was measured after 12 days under control conditions (untreated) or in the presence of 5.0-µM zeocin or bleomycin 0.2 µg/mL (n ≥ 20 roots for each condition). Data are from two independent experiments. Letters on the data indicate statistically different means (P < 0.05, ANOVA mixed model analysis, Tukey’s correction for multiple testing). See also Supplemental Data Set S1. C, Immunostaining of γH2AX foci accumulation (green) in nuclei stained with DAPI (blue) in Arabidopsis root tips from Col-0, atr-2 and atm-2 plants after 24 h of treatment with 5.0-µM zeocin. Two representative nuclei are shown for each line. Scale bar = 3 µm. D, Quantification of the number of γH2AX foci per nucleus in Col-0, atr-2, and atm-2 root tips after 24 h under control conditions (untreated) or in the presence of 5.0 µM zeocin. The number of nuclei analyzed for each genotype and condition is shown on each bar. For each sample, the γH2AX foci of analyzed nuclei were counted and grouped into six categories: no focus, 1–2, 3–5, 6–10, 11–20, and >20 foci per nucleus.