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. 2021 Jun 4;33(8):2662–2684. doi: 10.1093/plcell/koab158

Figure 5.

Figure 5

Accumulation of γH2AX foci in Zmatr and Zmatm root tips in response to HU and zeocin. A, Immunostaining of γH2AX foci accumulation (green) in nuclei stained with DAPI (blue) in maize root tips of WT plants and Zmatr-b and Zmatm-a mutants after 24 h of treatment with 5.0 mM HU or 150-µM zeocin. Scale bar = 5 µm. B, Quantification of the number of γH2AX foci per nucleus in ATR-B WT, ATM-A WT, Zmatr-b and Zmatm-a root tips after 24 h of treatment with HU (5 mM), or zeocin (150 µM). For each sample, the γH2AX foci of 100 nuclei were counted and grouped into five categories: 1–25, 26–50, 51–100, 10–200, and >200 foci per nucleus.