Figure 2 .

AUTS2-IPs and protein interaction network. (a) The top 20 AUTS2-IPs (ranked by S/L; see also Materials and Methods). RBPs in blue text; chromatin repressive proteins in red text; chromatin activating proteins in green text. (b, c) Double IF for AUTS2 (green) and NONO (magenta) in E13.5 (b) and P0.5 (c) neocortex. Sagittal sections. (d) IP-western blot (WB) of AUTS2 and NONO IPs, probed for NONO. (e) IP-WB of AUTS2 and NONO IPs, probed for AUTS2. (f) Interaction network of AUTS2-IPs as found by STRING-DB. One subnetwork (upper right) consisted of RBPs, including NONO and SFPQ (arrows); the other subnetwork (lower left) consisted of chromatin proteins. Scale bars: b1, 50 μm for b1, c1; 100 μm for b2, b3, c2, c3.