Figure 6 .

Normal structure but molecular defects in Auts2 cKO^ctx neocortex. (a, b) DAPI staining (inverted fluorescence) of P7 control (a) and Auts2 cKO^ctx (b) brains. Neocortical layers are numbered in (a). Coronal sections. Abbreviation: CC, corpus callosum. (c, d) IF to detect serotonin transporter (5-HTT) in same sections as (a, b). Arrowheads indicate 5-HTT+ layer 4 somatosensory barrels. Asterisks in (d): tissue artifacts. (e, f) ISH to detect Penk in P0 control (e) and Auts2 cKO^ctx (f) brains. Boxed areas are shown at higher magnification in insets. Arrowheads in (f) indicate cells with increased Penk expression, presumably C-R neurons. Sagittal sections. Abbreviations: FrCx, frontal cortex; OB, olfactory bulb. (g, h) IF to detect CTIP2 (gene Bcl11b) in P0.5 control (g) and Auts2 cKO^ctx (h) neocortex. Neocortical layers are numbered in (g). Pial to subplate binning is as indicated. Sagittal sections through somatosensory cortex. (i) Ctip2+ cells quantified across 9 binned images each for control and Auts2 cKO (wilcox-test P = 0.5321). Abbreviations: IZ, intermediate zone; SP, subplate. Scale bars: a, 500 μm for a–d; e, 500 μm for e, f; and g, 100 μm for g, h.