Figure 3.

RhBBX28 is a key regulator of petal senescence. A and B, Flower phenotypes (A) and length of the senescence phase (B) of WT, RhBBX28-RNAi, and RhBBX28-OX plants. Flower phenotypes were recorded once flowers were fully opened. Experiments were performed independently 3 times, with similar results. C, D, Relative expression levels of RhBBX28 (C) and H₂O₂ levels (D) in petals of WT, RhBBX28-OX #10, and RhBBX28-RNAi #23 rose flowers entrained under DD conditions for 3 days. For DD treatments, flowers were initially grown under LD conditions and then transferred to DD conditions. Experiments were performed independently twice, with similar results. One representative result is shown. Mean values ± sd are shown from three replicates (n = 3). White horizontal bars, day; black horizontal bars, night; gray horizontal bars, subjective day or night. Mean values ± sd are shown from seven replicates (n = 7) for each line. RhUBI2 was used as an internal control. Asterisks represent statistically significant differences (*P < 0.05; **P < 0.01), as determined by ANOVA. Scale bar, 1 cm.